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KMID : 1137820220430010001
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의공학회지
2022 Volume.43 No. 1 p.1 ~ p.10
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Effects of Micro-current Stimulation on lipid metabolism in Oleic Acid-Induced Non-Alcoholic Fatty Liver disease in FL83B cells
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Lee Han-A
Lee Min-Joo
Kim Han-Sung
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Abstract
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Non-alcoholic fatty liver disease(NAFLD) is excessive hepatic lipid accumulation mainly caused by obesity. This study aimed to evaluate whether micro-current stimulation(MCS) could modulate lipid metabolism regarding the Sirt1/AMPK pathway, fatty acid β-oxidation pathway, and lipolysis and lipogenesis-related factors in FL83B cells. For the NAFLD cell model, FL83B cells were treated with oleic acid for lipid accumulation. MCS were stimulated for 1 hr and used frequency 10 Hz, duty cycle 50%, and biphasic rectangular current pulse. The intensity of MCS was divided into 50, 100, 200, and 400 μA. Through the results of Oil red O staining, it was confirmed that MCSs with the intensity of 200 μA and 400 μA significantly reduced the degree of lipid droplet formation. Thus, these MCS intensities were applied to western blot analysis. Western blot analysis was performed to analyze the effects of MCS on lipid metabolism. MCS with the intensity of 400 μA showed that significantly activated the Sirt1/AMPK pathway, a key pathway for regulating lipid metabolism in hepatocytes, and fatty acid β-oxidation-related transcription factors. Moreover, it activated the lipolysis pathway and suppressed lipogenesis-related transcription factors such as SREBP- 1c, FAS, and PPARγ. In the case of MCS with the intensity of 200 μA, only PGC1α and SREBP-1c showed significant differences compared to cells treated only with oleic acid. Taken together, these results suggested that MCS with the intensity of 400 μA could alleviate hepatic lipid accumulation by modulating lipid metabolism in hepatocytes.
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KEYWORD
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Micro-current stimulation, Lipid metabolism, Hepatic lipid accumulation, Non-alcoholic fatty liver disease, FL83B cells
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